Bile acids. LV. 2,2-Dimethoxypropane: an esterifying agent preferred to diazomethane for chenodeoxycholic acid
نویسندگان
چکیده
Abbreviations and trivial names: chenodeoxycholate, 3a,7adihydroxy-5p-cholanate; TLC, thin-layer chromatography; PLC, preparative-layer chromatography; HPLC, high-pressure liquid chromatography; PMR, proton magnetic resonance spectroscopy; GLC, gas-liquid chromatography; MS, mass spectrometry. Address correspondence to William H. Elliott, E. A. Doisy Department of Biochemistry, St. Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, M O 63104. Shaw, R., and W. H. Elliott, unpublished observations. This paper reports the unequivocal identification of these side-products by comparison with the synthetic materials, and methods to reduce or eliminate their formation, thus providing an improved procedure for the derivatization of samples of chenodeoxycholic acid.
منابع مشابه
Bile acids. OV. 2,2-Dimethoxypropane: an esterifying agent preferred to diazomethane for chenodeoxycholic acid.
Artifacts formed by methylation of chenodeoxycholic acid with diazomethane are identical to the synthetic 3alpha- and 7 alpha-monomethyl ethers of methyl chenodeoxycholate. The extent of formation of these artifacts with time has been studied. No detectable artifacts were found with 2,2-dimethoxypropane as the methylating agent.
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